Roadkill hotspots of domestic mammals and wild mammals did not overlap. We detected roadkill aggregations for wild and domestic mammals. The wild mammals’ roadkill rate was 0.03 (± 0.02) individuals/km/day and the domestic roadkill rate was 0.02 (± 0.01). Of the 21 recorded mammal species, five were domestic. We found 482 mammals’ roadkills, including 260 (54 %) wild mammals, 164 (34 %) domestic and 58 (12.0 %) undetermined specimens. The monitoring was executed from April 2012 to March 2013, by car, at an average speed of 60 km/h, with two observers looking for roadkills on the highway. This study was carried out on the BR-050 highway, an area of Cerrado biome, in Southeastern Brazil. In addition, we investigate the influence of the landscape on the location of the aggregations. That is why we evaluate mammals’ roadkill aggregations in order to locate them and to determine if wild and domestic mammals’ roadkills overlap. Despite this, few studies consider domestic animals. Domestic animals are involved in a large number of traffic accidents and they represent danger to humans due to their size.
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